منابع مشابه
Properties and function of yeast pyruvate carboxylase.
Cannata and Stoppani (1, 2) found that bakers’ yeast contains an adenosine diphosphate-dependent phosphoenolpyruvate carboxykinase, and they ascribed the essential physiological role of oxaloacetate synthesis to it. Doubts concerning their interpretation arose, however, when it was revealed (3, 4) that cellfree preparations of the same microorganism could catalyze the direct carboxylation of py...
متن کاملDNA polymerases from bakers' yeast.
Two DNA polymerases are present in extracts of commercial bakers' yeast and wild type Saccharomyces cerevisiae grown aerobically to late log phase. Yeast DNA polymerase I and yeast DNA polymerase II can be separated by DEAE-cellulose, hydroxylapatite, and denatured DNA-cellulose chromatography from the postmitochondrial supernatants of yeast lysates. The yeast polymerases are both of high molec...
متن کاملFormaldehyde dehydrogenase from bakers' yeast.
In the course of studies of formaldehyde metabolism in yeast (I), an enzyme was found that catalyzed the oxidation of formaldehyde in the presence of glutathione and diphosphopyridine nucleotide. A similar enzyme was discovered independently by Strittmatter and Ball in liver (2). This paper describes the partial purification and some of the properties of yeast formaldehyde dehydrogenase. Some e...
متن کاملPyruvate Carboxylase from Chicken Liver
On the basis of initial velocity and product inhibition studies a nonclassical Ping Pong Bi Bi Uni Uni mechanism has been proposed for pyruvate carboxylase from chicken liver. The nonclassical feature of this mechanism is the proposal that each active site on the enzyme is composed of two separate and functionally distinct catalytic sites, i.e., a separate catalytic site exists for the reactant...
متن کاملCytoplasmic Methionyl - tRNA Synthetase from Bakers ’ Yeast
Methionyl-tRNA synthetase has been purified from a yeast strain carrying the MESl structural gene on a high copy number plasmid (pFL1). The purified enzyme is a monomer of M, = 85,000 in contrast to its counterpart from Escherichia coli which is a dimer made up of identical subunits (M, = 76,000; Dardel, F., Fayat, G., and Blanquet, S . (1984) J. Bucteriol. 160,1115-1122). The yeast enzyme was ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62681-x